[Cytometry] question about automated cell counters

Dennis J. Young djyoung at ucsd.edu
Tue Jan 26 11:44:09 EST 2010


For those of you like me trying to follow this thread, it's NOT Specter;
it's Scepter.
Looks cool.
<<http://www.millipore.com/cellbiology/cp1/scepter&cid=google-scepter?gclid=
CKnA1Li_wp8CFRD7agodinCA0Q>>

Dennis J. Young
Flow Cytometry Shared Resource
<<http://cancer.ucsd.edu/flow>>
Moores UCSD Cancer Center Room 2317-2Q
3855 Health Sciences Drive #0803
La Jolla CA 92093-0803
(858) 822-0407
 
-----Original Message-----
From: cytometry-bounces at lists.purdue.edu
[mailto:cytometry-bounces at lists.purdue.edu] On Behalf Of Stephen Kwok
Sent: Tuesday, January 26, 2010 8:19 AM
To: Brian McFarlin
Cc: cytometry at lists.purdue.edu
Subject: Re: [Cytometry] question about automated cell counters

I was just about to say I saw the Millipore last week.  A very spiffy 
little thing with disposable tips around $3 a pop.  Still, a nice 
handheld unit like a pipette, and can be put in any hood or BL2+ and 
cleaned down.  Also downloadable and easy to adjust the cell size for 
count.  Looks neat!

Steve

Brian McFarlin wrote:
> Not sure why I didn't think of this earlier, you may also want to look at
> the new Millipore Specter. It is a cool little handheld cell counter the
> size of a pipet. It can count within specific size ranges. I think it
costs
> around $2500, but you would have to ask a Millipore representative.
>
>
> On 1/26/10 9:43 AM, "Wiener, Doris" <dwiener at health.usf.edu> wrote:
>
>   
>> It seems a little excessive to buy a flow cytometer to do cell counts. I
>> looked into the Nexcelom and Invitrogen Countess and both will do the
job. My
>> preference at this point is the Nexcelom. It has a very nice display of
the
>> cells so you can review what has been counted for any artifact. It is
also
>> relatively inexpensive.
>>
>> Doris Wiener
>>
>> -----Original Message-----
>> From: cytometry-bounces at lists.purdue.edu
>> [mailto:cytometry-bounces at lists.purdue.edu] On Behalf Of Oliver Dienz
>> Sent: Monday, January 25, 2010 2:11 PM
>> To: Flow list
>> Cc: Jonathan.Boyson at uvm.edu
>> Subject: [Cytometry] question about automated cell counters
>>
>> We would like to purchase an automated cell counter potentially with
>> some flow cytometer capabilities (e. g. GFP, cell cycle, viability
>> etc.) and are looking for some input. I have so far found the
>> following models that
>> would more or less fit the bill:
>>
>> Guava Easycyte Mini
>> Beckman-Coulter Vi-cell
>> Partec CyFlow CCA or SL
>> Nexelcom Cellometer Vision
>>
>> There are a lot of positive posts about the Guava system. Does anyone
>> have experience with any of the others or one instrument that is not
>> included in the list?
>>
>> We are also interested in the operating costs of those instruments as
>> several of them seem to require proprietary (and therefore expensive)
>> reagents or materials. Has anyone made a cost per sample analysis for
>> any instrument? How is your experience with using homemade solutions
>> or reusing materials? Any info would be greatly appreciated.
>>
>> Thanks, Oliver
>>     
>
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