[Cytometry] flow cytometry of mouse BAL fluid
a.smith at centenary.usyd.edu.au
Wed Jan 27 06:08:40 EST 2010
We have a group here doing analysis of mouse BAL on a routine basis.
I asked the PhD student, Lauren McKnight, about her method and she said the following.
> A simple technique has been published by van Rijt et al. We use a similar method and find it works quite well.
> van Rijt et al (2004) A rapid flow cytometric method for determining the cellular composition of bronchoalveolar lavage fluid cells in mouse models of asthma. Journal of Immunological Methods 288(1-2):111-121"
> Lauren McKnight
> PhD Scholar, T Cell Biology Group
> Centenary Institute of Cancer Medicine and Cell Biology
Centenary Institute, Sydney, Australia
On 26/01/2010, at 12:39 AM, Ulf Gehrmann wrote:
> Dear all,
> I am interested in analysing and quantifying cells of mouse BALf. I
> would like to set up a panel distinguishing between macrophages,
> neutrophils, lymphocytes and eosinophils while running counting beads
> to approximate the cell number.
> Does anyone have experiences with mouse BALf and can you recommend a
> combination of antibodies?
> I appreciate any help or comments regarding this matter.
> Best wishes,
> Ulf Gehrmann
> Ulf Gehrmann, PhD-student
> Karolinska Institutet
> Department of Medicine, Solna
> Clinical Allergy Research Unit
> Karolinska Universitry Hospital Solna, L2:04
> 17176 Stockholm
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