[Cytometry] Advia or Isoton

Joanne Lannigan jl7fj at virginia.edu
Thu Feb 25 18:24:42 EST 2010

An even more economical choice would be to buy PBS concentrates and  
dilute them as needed. We use Biosure's 8X PBS solutions on all our  
instruments (including Caliburs) and have never had a problem. They  
have a preservative free version for sorting as well. Surprising that  
BD does not recommend the use of a competitors product :).

Joanne Lannigan, M.S.
Director, Flow Cytometry Core
University of Virginia
Jordan Hall Room 7065
1300 Jefferson Park Avenue
Charlottesville, VA 22908-0734
flowcytometry at virginia.edu
(434) 924-0274 Office
(434) 982-1071 Fax

On Feb 18, 2010, at 7:11 PM, Bruno Verbeno wrote:

> Dear flow cytometry community
> We have a three colour FACSCalibur at our lab. But at the end of  
> last year
> we just can`t buy the ADVIA sheath fluid. As we also have a Beckman  
> couter
> cell counter we also have the ISOTON sheath fluid. At my previous  
> lab we
> used to use  ISOTON in FACSCalibur, but BD does not recommend the  
> use. Does
> anyone use ISOTON instead of the BD recommended sheath fluids?
> Best regards
> Bruno Verbeno
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