[Cytometry] Propidium staining

snehal mhatre snehalrm at gmail.com
Tue Feb 9 23:17:28 EST 2010

I have to stain my cell line (K562) with PI (Propidium Iodide) to check the
viability. I am adding 2uL of PI and incubating it at Room temperature for
3mins and the aquiring on flow cytometer without washing. The peak is
shifting. So, i tried it with freshly isolated human PBMC but I am getting
the same results.
I even tried giving it a wash before aquiring, but it didnt made any change.

I m aquiring PI in FL3.

Can anyone help me out with this? I wanted to know is my protocol is right
or can anyone suggest me any changes.

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