[Cytometry] Aria II

Tennant, Richard R.Tennant at exeter.ac.uk
Thu Feb 4 11:33:34 EST 2010


Hello

Do people using the embedded O-ring nozzle on the Aria II get a much improved stream than when using the previous separate O-ring and nozzle? Is it worth buying a new nozzle for?

Many Thanks

Richard

-----------------------------------------------------
Richard Tennant
Research Technician - Flow Cytometry
University of Exeter
School of Biosciences
Mezzanine Lab
Geoffrey Pope Building
Stocker Road
Exeter
United Kingdom
EX4 4QD







On 1 Feb 2010, at 20:25, Barsky, Lora wrote:

I'd like to comment on Question 1.  I've been using an Aria I for four months.

Regarding the embedded O-ring nozzles,  there are a few things to keep in mind.
A) Never user Contrad to clean this type of nozzle if there is a clog.  Reply on other things, from H20 to FACSClean (10% bleach) to FACSRinse (detergent) and sonication.
B)  Replace an embedded Nozzle  with a damaged one, when doing a Flow Cell Clean (with Contrad), which you might find yourself doing 1x month when CST fails to "find the beads".
C) You are able to use the nozzle O-rings from the Aria I, if and when your Aria II nozzle looses it's embedded o-ring.  Keep in mind, the o-rings will be subject to "wear and tear" similar to what you had with the Aria I.   If you're removing and resetting an Aria II nozzle, with each shutdown, you may blow through the supply quickly.
D) If you want to replace a damaged, embedded nozzle, talk with your instrument rep to see if discounts are available prior to placing your order.  My sales rep mentioned the prices will be coming down.
E)  I've been three months with an embedded nozzle and prefer it now, over the older nozzle type.  There is little to no "messing with" that I have to do to get a perfect stream (no satelites!!)

Good Luck,

Lora
On Feb 1, 2010, at 8:45 AM, Marcos E. García-Ojeda wrote:

Hello everybody.

We recently upgraded our old ARIA I to an ARIA II.  However, I have a
couple of questions that I would like to pass by you. I will appreciate
any information that you can provide me.

1) O-ring:  The new nozzle comes with a fixed o-ring on it.  If the
o-ring gets damaged, the manual says that the seal can be temporarily
changed. This implies that you have to buy new nozzles when the original
ones get damaged?

2) Nozzle sterilization: The aseptic set up doesn't mention how to
sterilize the nozzle for  aseptic sorts, just how to clean it to remove
particles by sonication.  What can be done to sterilize the nozzle and
not damage the fixed o-ring seal?

3) Pressure probe:  While the sheath tank can be autoclaved, the
pressure probe cannot.  The manual says to put it in ethanol to
sterilize it.  BD tech services recommended 10% bleach.  What do you
recommend to clean the pressure probe before sterile sorts?

4) Water tank:  The ARIA II manual advices to add 3ml of bleach/L water
during sterile sorts.  I was advised just to autoclave the water and
recevoir.  I would like to hear what other users do with their water
reservoir to avoid contamination.

5) Sheath filter.  The instruction for sterilizing the instrument
indicate to put a new sheath filter every time you prepare for aseptic
technique.  Are you changing the filter everytime?  Do you have a filter
designated exclusively for sterile sorts?

Any kind of advice will be very helpful.  Thanks for your help!

Marcos
--

Marcos E. García-Ojeda, PhD

Assistant Professor

School of Natural Sciences

University of California, Merced

5200 North Lake Road

Merced, CA 95343 USA

209-228-6986 Office

209-337-8279 Cell

209-228-4053 Fax



http://qsb.ucmerced.edu/faculty/mgarcia-ojeda/lab/

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Lora W. Barsky
Manager, Flow Cytometry Core
Eli and Edythe Broad Center for Stem Cell and Regenerative Medicine
1450 Biggy St, HNRT4513
MC 9601-NRTLG591
Los Angeles, CA  90033
phone (323) 442-7942
lbarsky at usc.edu<mailto:lbarsky at usc.edu>
Sort Appointments: facscore at gmail.com




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