[Cytometry] Aria II
"Marcos E. García-Ojeda"
mgarcia-ojeda at ucmerced.edu
Mon Feb 1 11:45:50 EST 2010
We recently upgraded our old ARIA I to an ARIA II. However, I have a
couple of questions that I would like to pass by you. I will appreciate
any information that you can provide me.
1) O-ring: The new nozzle comes with a fixed o-ring on it. If the
o-ring gets damaged, the manual says that the seal can be temporarily
changed. This implies that you have to buy new nozzles when the original
ones get damaged?
2) Nozzle sterilization: The aseptic set up doesn't mention how to
sterilize the nozzle for aseptic sorts, just how to clean it to remove
particles by sonication. What can be done to sterilize the nozzle and
not damage the fixed o-ring seal?
3) Pressure probe: While the sheath tank can be autoclaved, the
pressure probe cannot. The manual says to put it in ethanol to
sterilize it. BD tech services recommended 10% bleach. What do you
recommend to clean the pressure probe before sterile sorts?
4) Water tank: The ARIA II manual advices to add 3ml of bleach/L water
during sterile sorts. I was advised just to autoclave the water and
recevoir. I would like to hear what other users do with their water
reservoir to avoid contamination.
5) Sheath filter. The instruction for sterilizing the instrument
indicate to put a new sheath filter every time you prepare for aseptic
technique. Are you changing the filter everytime? Do you have a filter
designated exclusively for sterile sorts?
Any kind of advice will be very helpful. Thanks for your help!
Marcos E. García-Ojeda, PhD
School of Natural Sciences
University of California, Merced
5200 North Lake Road
Merced, CA 95343 USA
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