[Cytometry] cell cycle analysis
W.E.Corver at lumc.nl
Sat Nov 28 04:25:57 EST 2009
No attachment for me!
Do you use a reference?
Are you using cultered cells?
Maybe the "prominent" peak is a 2n G1 peak and the second peak is your 4n G1 population and you are dealing with endoreduplication.
Van: cytometry-bounces at lists.purdue.edu namens Huang, Vera
Verzonden: vr 11/27/2009 20:50
Aan: cytometry at flowcyt.cyto.purdue.edu
Onderwerp: [Cytometry] cell cycle analysis
I have been doing some cell cycle analysis by PI staining and noticed the appearance of a prominent peak on the left of the G1 peak in my cells. It does not seem to look like a subG1 peak or cell debris. Does anyone know what this peak represents? (see attached)
Thank you for your time.
Vera Huang, Ph.D.
Long-Cheng Li Lab
University of California, San Francisco
Email: huangv1 at urology.ucsf.edu <mailto:huangv1 at urology.ucsf.edu>
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