[Cytometry] cell cycle analysis

W.E.Corver@lumc.nl W.E.Corver at lumc.nl
Sat Nov 28 04:25:57 EST 2009


Vera,
 
No attachment for me!
Do you use a reference?
Are you using cultered cells?
Maybe the "prominent" peak is a 2n G1 peak and the second peak is your 4n G1 population and you are dealing with endoreduplication.
 
Regards,
 
Willem Corver

________________________________

Van: cytometry-bounces at lists.purdue.edu namens Huang, Vera
Verzonden: vr 11/27/2009 20:50
Aan: cytometry at flowcyt.cyto.purdue.edu
Onderwerp: [Cytometry] cell cycle analysis



Hi all,

I have been doing some cell cycle analysis by PI staining and noticed the appearance of a prominent peak on the left of the G1 peak in my cells. It does not seem to look like a subG1 peak or cell debris.  Does anyone know  what this peak represents? (see attached)

Thank you for your time.

Vera

Vera Huang, Ph.D.
Postdoctoral Fellow
Long-Cheng Li Lab
University of California, San Francisco
Email: huangv1 at urology.ucsf.edu <mailto:huangv1 at urology.ucsf.edu>

_______________________________________________
Cytometry mailing list
Cytometry at lists.purdue.edu
https://lists.purdue.edu/mailman/listinfo/cytometry




More information about the Cytometry mailing list