[Cytometry] Lysing fixed cells?

Gary A. Ward Gary.A.Ward at Dartmouth.EDU
Tue Nov 24 10:46:52 EST 2009


Hello Flow-ers,

I have a user that is interested in lysing some fixed cells that didn't lyse well enough the first time.  She wants to get rid of the red blood cells without damaging the monocytes.  Is this possible? Has anyone ever done this?  Any ideas?

Please respond to me and I'll pass the word along to her.  Thank You!

Gary A. Ward
H C Englert Cell Analysis Laboratory
Norris Cotton Cancer Center
Lebanon, NH USA



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