[Cytometry] Compensation LSRII

Ian Dimmick ian.dimmick at newcastle.ac.uk
Tue Nov 24 08:11:39 EST 2009


The compensation values will be proportional to the filter values and tolerances which will be individual for each instrument type, as well as the different PMT gains from instrument to instrument  , the Calibur relies on a 670lp filter for PerCp (LSR II 695/40) and 585/42 for PE (LSR II 575/26)so you should not expect to get the same values as the spill over detection is different .

Regards

Ian
Ian Dimmick
Flow Cytometry Core Facility Manager
Institute of Human Genetics
Bioscience Centre
International Centre for life
Newcastle Upon Tyne
NE1 3BZ
UK
Ian.Dimmick at ncl.ac.uk
Tel 0044 191 2418831
Fax 0044 191 2418666
(mob) 0044 7970344823
http://www.ncl.ac.uk/ihg.
Flow cytometry calendar
 (http://flowcytometry.calendarhost.com)
 

-----Original Message-----
From: cytometry-bounces at lists.purdue.edu 
[mailto:cytometry-bounces at lists.purdue.edu] On Behalf Of Sarah Maurice
Sent: 24 November 2009 12:10
To: cytometry at lists.purdue.edu
Subject: [Cytometry] Compensation LSRII


 

To all flowers

When performing autocompensation on our LSRII we have noticed 
values up to 45% when analyzing PerCP  (dichroic LP 685 - BP 
695/40) and PE (dichroic LP 550 - BP 575/26) together. These 
values are much greater than those seen on our Caliber and 
AriaII. Experts in compensation, any thoughts on the subject??

 

Sarah Maurice, PhD

Flow Cytometry - Infrastructure unit 

Life-Sciences and Engineering 

Technion - Israel Institute of Technology

Haifa, Israel, 32000

Tel: 972-4-829 5131

Fax: 972-4-829 5131

Email: msarah at tx.technion.ac.il

Web: Http://ISU.technion.ac.il <http://isu.technion.ac.il/> 

 

 

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