[Cytometry] Accudrop fluorochrome: more

Larry W. Arnold lwarma at med.unc.edu
Thu Nov 19 17:25:08 EST 2009


Geoff

Check this out - http://www.spherotech.com/DropDelay.htm

Larry

At 05:07 PM 11/19/2009, you wrote:
>Hi All,
>Thanks for the numerous replies, none of which have answered the 
>question BTW. Despite the popularly held misconception which even 
>appears in print (thanks Mark) for example
>
>http://www.jimmunol.org/cgi/content/full/174/11/7226
>
>Accudrop beads are not doped with APC, but a fluorochrome which 
>emits much further out in the red around 750 and beyond (check the 
>TDS). Fairly obviously, BD would view this as company confidential 
>information, and righty so.
>However, for a project I have running I need to find a spectral 
>matched fluorochrome to the Accudrops. If one scans the literature 
>there are few fluorochromes which have a sufficiently large Stokes 
>shift and are as broad their emission profile as whatever is loaded 
>in the Accudrops, thus I remain in ignorance on this one....
>
>Thanks again,
>Geoff
>
>________________________________________
>From: cytometry-bounces at lists.purdue.edu
>[cytometry-bounces at lists.purdue.edu] On Behalf Of Geoffrey Osborne
>[g.osborne at uq.edu.au]
>Sent: Thursday, 19 November 2009 10:02 AM
>To: cytometry at flowcyt.cyto.purdue.edu
>Subject: [Cytometry] Accudrop fluorochrome
>
>Hi All,
>
>Would someone happen to be able to tell me what fluorochrome BD's
>Accudrop beads are labeled with? Strangely enough I can't seem to find
>it on the bottle....
>
>
>
>Thanks in advance,
>
>
>
>Geoff
>
>--
>Geoffrey Osborne
>Director of Flow Cytometry,
>The Queensland Brain Institute /Australian Institute for Bioengineering
>and Nanotechnology,
>The University of Queensland,
>St Lucia, QLD, 4072, Australia
>Ph (61) 07 33466397
>email g.osborne at uq.edu.au
>Please consider the environment before you print anything!
>
>
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Larry W. Arnold, Ph.D.
Research Professor and Director, Flow Cytometry Facility
Department of Microbiology and Immunology
Rm. 602 Mary Ellen Jones  CB# 7290
University of North Carolina
Chapel Hill, NC 27599
Phone: 919-966-1530
FAX: 919-843-5624
http://flowcytometry.med.unc.edu




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