[Cytometry] Bovine WBC analysis by FCM

Gabriele Grunig grunig01 at nyu.edu
Wed Mar 4 09:54:21 EST 2009


Hi Kathy,

You might want to contact Bettina Wagner (bw73 at cornell.edu; phone +1 607 253 3813; fax: +1 607 253 3440) at Cornell University in Ithaca.  Bettina is a member of the US Veterinary Immune Reagent Network.

Hope this helps !

Gabi Grunig


_________
Gabriele Grunig, DVM, PhD
Department of Environmental Medicine
NYU School of Medicine
57 Old Forge Rd.
Tuxedo NY 10987
email: grunig01 at nyu.edu
phone: 845-731-3669


----- Original Message -----
From: Kathy Heel <kathy.heel at uwa.edu.au>
Date: Tuesday, March 3, 2009 11:41 pm
Subject: [Cytometry] Bovine WBC analysis by FCM
To: cytometry at flowcyt.cyto.purdue.edu

> Hello All,
> 
>  
> 
> I have a researcher looking at the tropism of B cells in cattle with
> lentiviral infection. Does anyone have any experience working with
> bovine WBCs, especially fixation and storage? Samples will be taken from
> infected cattle in Bali over a 4 week period and the WBCs washed, fixed
> and stored prior to transport back to Australia for FCM analysis of
> lymphocyte populations. I'm looking for a simple robust wash/fix/freeze
> (?) protocol as the labs in Bali is fairly basic. Any tips on staining
> (CD4, CD8, CD17, CD21) and flowing bovine cells also greatly
> appreciated.
> 
>  
> 
> Many thanks
> 
> Kathy
> 
>  
> 
> Kathy Heel PhD
> 
> Lecturer
> 
> Centre for Microscopy, Characterisation and Analysis (M510)
> 
> The University of Western Australia
> 
> 35 Stirling Highway
> 
> Crawley WA 6009
> 
> Australia
> 
>  
> 
> Ph 61 8 9346 4525
> 
> Fax 61 8 9346 3469
> 
> Mob 0416 085 960
> 
> www.cmca.uwa.edu.au
> 
>  
> 
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