[Cytometry] acceptance criteria for cell cycle analysis

Verity Software verity at vsh.com
Fri Jul 31 11:08:03 EDT 2009

Hello Jose

As the title indicates, those guidelines were established for Clinical

There has been quite a bit of work since those guidelines were published to
standardize the approach to Clinical DNA Analysis.  I have listed some
references below.

If you have some specific questions I would be happy to answer them

Best regards,
Donald J. Herbert
Technical Support Manager
Verity Software House
tech at vsh.com


DNA Consensus Report:  Cytometry Vol 14, No.5 '93, Wiley-Liss

Theoretical Aspects of Data Analysis, Chapter 3 (pp 41-61), in Clinical Flow
Cytometry: Principles and Application, Edited by Kenneth D. Bauer, Riccardo
E. Duque, T. Vincent Shankey. Pub by Williams and Wilkins, 1993

REVISITED, by C. Bruce Bagwell MD, PhD, Gary M. Clark, PhD, Frederique
Spyrantos, DrSc; et al: in New Applications of Flow Cytometry, pp 875-895,
vol 21 Number 4, December 2001, Published by Clinics in Laboratory Medicine

Optimizing Flow Cytometric DNA Ploidy and S-Phase Fraction as Independent
Prognostic Markers for Node-Negative Breast Cancer Specimens; by C. Bruce
Bagwell MD, PhD, Gary M. Clark, PhD, Frederique Spyrantos, DrSc; et al: in
Cytometry (Communications in Clinical Cytometry) 46:121-135 (2001) Published
by Wiley-Liss, Inc.

Doublet Discrimination in DNA Cell-Cycle Analysis Wersto etal. Cytometry
(Comm. Clin.Cytometry) 46:296–306, 2001.

Paraffin DNA

Overton WR and McCoy JP: Reversing the Effect of Formalin on the Binding of
Propidium Iodide to DNA. Cytometry 16(4):351-356, 1994.

Overton WR, Catalano E, and McCoy JP: Method to Make Paraffin-embedded
Breast and Lymph Tissue Mimic Fresh Tissue in DNA Analysis. Cytometry
26(2):166-171, 1996.

Douwes Dekker PB, Corver WE, Hogendoorn PC, van der Mey AG, and Cornelisse
CJ: Multiparameter DNA flow-sorting demonstrates diploidy and SDHD wild-type
gene retention in the sustentacular cell compartment of head and neck
paragangliomas: chief cells are the only neoplastic component. J Pathol
2004, 202: 456-462

Glogovac JK, Porter PL, Banker DE, and Rabinovitch PS: Cytokeratin labeling
of breast cancer cells extracted from paraffin-embedded tissue for bivariate
flow cytometric analysis. Cytometry 1996, 24: 260-267

Jordanova ES, Corver WE, Vonk MJ, Leers MP, Riemersma SA, Schuuring E, and
Kluin PM: Flow cytometric sorting of paraffin-embedded tumor tissues
considerably improves molecular genetic analysis. Am J Clin Pathol 2003,
120: 327-334

Leers MPG, Schutte B, Theunissen PHMH, Ramaekers FCS, and Nap M: Heat
pretreatment increases resolution in DNA flow cytometry of paraffin-embedded
tumor tissue. Cytometry 1999, 35: 260-266

-----Original Message-----
From: cytometry-bounces at lists.purdue.edu
[mailto:cytometry-bounces at lists.purdue.edu] On Behalf Of jose estevam
Sent: Wednesday, July 29, 2009 4:50 PM
To: cytometry at lists.purdue.edu
Subject: [Cytometry] acceptance criteria for cell cycle analysis

Hi everyone,

I'm trying to understand what the current guidelines are for cell cycle
analysis using Modfit.  From what I understand, the majority still rely on
Shankey, et al paper "Guidelines for the implementation of clinical DNA
cytometry".  Have there been any updates to this analysis?  

Thanks for your help,

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