[Cytometry] Targeting RBC

John Mario González Escobar johgonza at uniandes.edu.co
Tue Jul 28 14:59:08 EDT 2009



John M. Gonzalez MD, PhD
Biomedical Sciences, School of Medicine
Universidad de los Andes, Bogota DC, Colombia
Carrera 1 No. 18A-10 piso 8 Edifico Q
Phone: 57 (1) 3394949 ext 3718 or 3900 (lab)
Fax: 57 (1) 3324281

-----Mensaje original-----
De: cytometry-bounces at lists.purdue.edu
[mailto:cytometry-bounces at lists.purdue.edu] En nombre de Dave Adams
Enviado el: lunes, 27 de julio de 2009 03:20 p.m.
Para: cytometry at flowcyt.cyto.purdue.edu; Dave Adams
Asunto: Re: [Cytometry] SE Troubleshooting issue

Nevermind, pulled some old prints, traced some signals and we're back in the
saddle.

Dave

>>> "Dave Adams" <davadams at med.umich.edu> 07/27/09 4:13 PM >>>
Greetings!  I have a bit of a vantage troubleshooting issue that I'd
appreciate your help on.

The short version:

I know longer have computer control of the dead times/laser delays on two of
my vantages.  Can't seem to puzzle it out.

The long version:

We had one vantage suddenly lose the ability to "see" signals real or
generated (test).  Boards were swapped and engineers consulted and the
problem ended up being the peak and hold card (now replaced).  However, now
neither the original problematic vantage nor the machine that was acting as
card donor has computer control of the delays.  They can be set manually via
the three pots on the peak and hold card.

The software, of course, thinks that the computer still has control and you
can really ramp up the abort rate if you don't set the computer to match the
manually dialed in time delays.

So, as the problem occurred while card swapping, I initially thought that we
had hooked a cable up wrong (always possible... heck we have omnicomps on
these things), so we checked them against the other two vantages and all
looks well.  Cards are also all fully seated.  If anyone has seen this
before or has any thoughts, I'd appreciate hearing them.  Thanks.

David Adams
University of Michigan
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