[Cytometry] single-cell GFP sorting references

Nebe-Von-Caron, G g.nebe-von-caron at invmed.com
Tue Jul 21 12:46:47 EDT 2009


Depending on your instrument performance and the size of the bugs you shoul=
d ideally sort from forward scatter versus green fluorescence as expression=
 is volume dependent. You will then be able to sort cells with the same exp=
ression intensity but different forward scatter as shown in the example fro=
m my tutorial or cells with different intensity but similar scatter. Howeve=
r if the investigator uses a plasmid, the expression intensity is less info=
rmative then using a single gene copy. Ideally you would want to measure ag=
ainst DNA content but the cells might not be too happy about that.


Regards

Gerhard


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