[Cytometry] PNH

Tony Bakke bakkea at ohsu.edu
Mon Jul 20 19:42:47 EDT 2009


We see about one patient positive for a PNH clone out of 10 with anemia,
cytopenia, or suspected PNH.  The size of the clones vary from 0.5 to
90%, with most between 0.5 and 10%.  You should be able to find some low
level positives.  These low levels are not considered diagnostic for
PNH, but the exact cutoff for PNH is not yet defined.  If you have
problems, contact me.

You were correct to stop using the RedQuant.  The attached paper shows
that labs testing for CD55 and CD59 on RBCs only have both high false
positive and false negative rates.

Tony Bakke, PhD, D(ABMLI)
Scientific Director, Special Immunology and Clinical Flow Cytometry
Department of Pathology
Oregon Health & Science University

-----Original Message-----
From: cytometry-bounces at lists.purdue.edu
[mailto:cytometry-bounces at lists.purdue.edu] On Behalf Of Katzke,
Kathleen A.
Sent: Friday, July 03, 2009 6:05 AM
To: cytometry at lists.purdue.edu
Subject: [Cytometry] PNH

I have read the article "Diagnosing PNH with FLAER and Multiparameter
Flow Cytometry" by R. Sutherland. We are starting a method development
for PNH. We used to use the RedQuant and CellQuant kits for testing
years ago, but quit as I couldn't get the support I needed and then the
results weren't coming out well either after years of testing different
methods and finally bringing the kits live. So, we quit that testing
after 5 months.
My question is, with using the procedure from the article, we had
thought of doing 20 samples of patients with anemia or those samples
that are sent out for PNH testing for comparison. We only sent out 16
samples last year so don't have a lot of reference work for this test. I
was wondering what is recommended for testing and comparisons for this
test. I realize we will rarely see a positive PNH patient, esp. with our
initial testing, but wanted some feedback on how to proceed.      Thanks
for all your help.

Kathy Katzke, MT (ASCP) QCym 
Flow Cytometry 
OSF System Lab 
1224 Berkley St
Peoria, IL 61637 
work 309-624-9036 
fax 309-624-9037

kathleen.katzke at osfhealthcare.org 



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