[Cytometry] question about antibodies stability

Sharma, Parul Parul.Sharma at childrens.harvard.edu
Mon Jul 20 16:47:58 EDT 2009

Hi Paul,
totally agree with Steve,
We had been using the antiRANK antibody fromX a company and all was working fine until one day they stopped working. Luckily we had a tube of the old antibody and everything including the lot numbers matched. Finally thru a long discussion we figured the antibody was losing potency because it was shipped in a different packaging system.You guessed right they were shipping it at room temperature when they had been shipping it on dry ice earlier......
I asked them to ship this antibody again frozen this time and it worked.
We have seen this with other antibodies too! However some antibodies are robust!
I wish we could do something about this. We have the same issue with some of our western blot developers too!
Sometimes replacements just do not cut it with precious samples!

-----Original Message-----
From: cytometry-bounces at lists.purdue.edu [mailto:cytometry-bounces at lists.purdue.edu] On Behalf Of McClellan, Steve
Sent: Monday, July 20, 2009 9:25 AM
To: cytometry at lists.purdue.edu
Subject: Re: [Cytometry] question about antibodies stability

Hi Paul,
Being in Florida, I have always had concerns with companies that do not ship cold.  If it is 90-100 outside, you can bet that the inside of that delivery truck is at least 120!  Over the years, I would estimate that about half of the products I have received this way did not work.  I now refuse to buy from companies that do not ship on a cold pack.  If they say their products are stable at room temperature I want to see the stability studies done at 120.  Of course they are happy to replace them, after you waste your precious clinical samples finding out that they DID indeed get fried in transit.  I advise caution. Kind regards, Steve

Steve McClellan
Sr. Biological Scientist
Interdiciplinary Center for Biotechnology Research(ICBR)-
Flow Cytometry Core Laboratory
University of Florida
352-273-8185 (office)
352-273-8070 (fax)
smcclell at biotech.ufl.edu
Cancer/Genetics Research Complex-Room 292A

-----Original Message-----
From: cytometry-bounces at lists.purdue.edu [mailto:cytometry-bounces at lists.purdue.edu] On Behalf Of J. Paul Robinson
Sent: Sunday, July 19, 2009 9:27 AM
To: Cytometry Mailing List; J Paul Robinson
Subject: [Cytometry] question about antibodies stability

Dear colleagues -

I recently had about $800 antibodies shipped for a series of
experiments. They were shipped on Saturday and the temperature over the
weekend was nearly 100 degrees...when they were delivered on Monday
morning they were warm. I wrote to the company and got the following
response. I have removed the name of the company. The cost of doing the
experiments is very high and will use valuable clinical materials. The
company told staff that they would replace the antibodies if they didn't
work. When we questioned them here is their response - I'd like to hear
of others think this is accurate or acceptable.

---------company response to my email-----------------
"We understand your frustration. I would like to reassure you that we
don't ship any orders out on Fridays. Our standard method of shipping is
to ship Saturday evening to reduce the amount of time spent in transit.
Also, we have successfully shipped Saturday for Monday delivery for the
past three years, which is why we guarantee that our products will be
stable when they arrive.

Please read below for more information {website removed]:

All [company removed] products are thawed to room temperature before
shipping and all are shipped out at room temperature every day of the week.

Please note that the majority of antibodies are stable at room
temperature (RT) for long periods of time. Experiments conducted at
[company removed] show that, for antibodies left at RT, activity remains
constant for 2 months before showing any decline. However, as a
precautionary measure and to extend the shelf life of the antibodies, we
ship our antibodies in refrigerated packaging which has been shown to
provide a stable environment for antibodies in transit."

So do I risk my valuable samples to antibodies that arrive under these
conditions? I am really not sure - perhaps my concerns are not warranted??


Paul Robinson

J. Paul Robinson
SVM Professor of Cytomics
Professor of Biomedical Engineering
Director, Purdue University Cytometry Laboratories Past-President, International Society for Analytical Cytology (now International Society for Advancement of Cytometry)

Purdue University Cytometry Laboratories
Bindley Bioscience Center
1203 West State Street
Discovery Park, Purdue University
West Lafayette, IN 47907-2057
Ph (765) 494 0757; Fax (765) 494 0517
email: jpr at flowcyt.cyto.purdue.edu

Track Paul's climbs in the Himalayas - Everest - April-May 2009 http://www.cyto.purdue.edu/trackpaul/
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