[Cytometry] Clinical Flow Course

PK Wallace pkwallace at gmail.com
Fri Jul 17 08:53:11 EDT 2009

Hi Everyone,
The The Twenty First Annual Clinical Course on Flow & Image Cytometry will
be held again this year at the Roswell Park Cancer Institute in Buffalo, New
York.  As in past years, the Course includes hands-on laboratory and
computer work in small groups led by an expert faculty, lectures on a
variety of general and advanced topics, and many opportunities for informal
discussion.  The Course  emphasizes basic flow theory, current best
practices in the clinical laboratory, and the diagnosis of leukemia &

Specific topics that will be covered include:
leukemia and lymphoma phenotyping and interpretation of histograms;
analysis of myeloid disorders & granulocyte function;
analysis of intracellular epitopes and signaling pathways in leukemia;
staining and histogram analysis for DNA cell cycle stage and ploidy;
high level multicolor clinical flow cytometry
quality control, standardization and proper experimental design;
flow cytometric diagnosis of primary immunodeficiencies;
reference and alternative methods for CD4 and CD34 measurements;
selection of fluorochromes and compensation issues;
staining of leukocytes with tetramers to evaluate antigen specificity;
use of tracking dyes to follow cell proliferation;
imaging techniques for chemosensitivity & FISH analysis.

For more information or to register goto:
or contact me directly, clincourse at pricflow.org


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