[Cytometry] CS&T

Felix Heymann felix.reichel at ukb.uni-bonn.de
Fri Jul 17 19:10:52 EDT 2009

Hi Pam,
I just came back from an Aria II training from BD today (lucky me, the 
guys did a real terrific job!) and of course we did a lot of baseline 
configs and performance checks to calibrate the instruments. Our 
trainers actually stressed that the bead lot no is quite important, 
since there are quite some informations (I guess mostly about the actual 
fluorescent properties of the specific lot)  hidden in the lot files 
sitting on your instrument. If there's new bead lots out which have not 
been listed on your instrument, you should actually also download the 
actual information from the BD website. Since the machine uses the CS&T 
to set also your laser delays  and area scalings, not only the PMTs, 
working with the wrong lot no actually could affect the accurate digital 
allignment of your instrument, so from my point of view it's quite 
necessary to stick to the lot no if you run a performance check 
belonging to a certain baseline. If you do regular performance checks, 
you can also keep track about the development of your intrument 
performance and can see early if e.g. a laser starts to get bad. Since 
core facilities have a high throughput of external customers, regular 
quality controls guarantee a high level of performance from your core 
and keep customers happy......and in case things go wrong you can at 
least prove that it was not due to a problem with the machines.


> Hi Pam,
> We have multiple "configurations" of the instrument with the different  
> bead lots.  I ran separate baselines for the users.  The user just has  
> to make sure to pick the right configuration and can do the  
> performance checks as needed.  Please let me know if you need any  
> further details.
> Good luck,
> Neal
> ICBR Flow Cytometry Facility
> University of Florida
> On Jul 6, 2009, at 2:10 PM, Moody, Pamela wrote:
>> Hi Everyone,
>> Our core facility has an Aria I, Aria II and LSRII which are all  
>> running
>> CS&T beads.  We have trained users which are allowed to run these
>> instruments and run the CS&T beads.  Each of these users has a  
>> different
>> lot number of CS&T beads.  I would like to know what other core
>> facilities do in this situation.  Can you run different bead lots with
>> the same baseline?  Do you ignore the bead lot?  New users need to do
>> everything by the book and exactly how they were shown in class.  They
>> don't always know what it means if you ask them but they want to do it
>> anyway.  I could go on forever about this but I would rather hear your
>> stories.
>> Thanks
>> Pam
>> Pamela Moody
>> Manager, Flow Cytometry Shared Resource
>> Cold Spring Harbor Laboratory
>> Hershey Building
>> One Bungtown Road
>> Cold Spring Harbor, New York 11724
>> Phone: (516) 367-8477
>> Fax: (516) 367-8461
>> Website: http://www.cshl.edu/cancercenter/shared_resources.html
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