[Cytometry] Compensation with liver cells

SIMON MONARD smonard at staffmail.ed.ac.uk
Fri Jul 10 13:23:58 EDT 2009


Oops

That should have read,"6 micron carboxylated POLYSTYRENE beads are a  
better choice for larger cells". You can also couple them to GFP which  
is handy.
Simon

Quoting Mario Roederer <roederer at drmr.com>:

> No... antibody capture beads are not designed with a particular cell
> subset in mind.  Any compensation bead will work for any cell type, as
> long as the positive beads are brighter (or at least as bright) as
> your stained cells.
>
> Adding background to your beads (or using beads with more background)
> will only decrease the precision (although not the accuracy) of your
> compensation.
>
> mr
>
>
> On Jul 10, 2009, at 5:24 AM, SIMON MONARD wrote:
>
>> You will likely find them unusable as the commercial ones are designed
>> for use with lymphocytes. If you are planning on doing much FACS then
>> I suggest you make your own capture beads using beads with a
>> background fluorescence comparable to your unstained cells
>
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>



Simon Monard
FACS Facility Manager
MRC Centre for Regenerative Medicine
Institute for Stem Cell Research
University of Edinburgh
Roger Land Building
West Mains Road
Edinburgh
EH9 3JQ

Tel. Lab 0131 6505876
Tel Office 0131 6517265

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