[Cytometry] Compensation with liver cells

SIMON MONARD smonard at staffmail.ed.ac.uk
Fri Jul 10 13:23:58 EDT 2009


That should have read,"6 micron carboxylated POLYSTYRENE beads are a  
better choice for larger cells". You can also couple them to GFP which  
is handy.

Quoting Mario Roederer <roederer at drmr.com>:

> No... antibody capture beads are not designed with a particular cell
> subset in mind.  Any compensation bead will work for any cell type, as
> long as the positive beads are brighter (or at least as bright) as
> your stained cells.
> Adding background to your beads (or using beads with more background)
> will only decrease the precision (although not the accuracy) of your
> compensation.
> mr
> On Jul 10, 2009, at 5:24 AM, SIMON MONARD wrote:
>> You will likely find them unusable as the commercial ones are designed
>> for use with lymphocytes. If you are planning on doing much FACS then
>> I suggest you make your own capture beads using beads with a
>> background fluorescence comparable to your unstained cells
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Simon Monard
FACS Facility Manager
MRC Centre for Regenerative Medicine
Institute for Stem Cell Research
University of Edinburgh
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