[Cytometry] CS&T

Neal Benson nbenson at ufl.edu
Wed Jul 8 21:14:35 EDT 2009

Hi Pam,

We have multiple "configurations" of the instrument with the different  
bead lots.  I ran separate baselines for the users.  The user just has  
to make sure to pick the right configuration and can do the  
performance checks as needed.  Please let me know if you need any  
further details.

Good luck,

ICBR Flow Cytometry Facility
University of Florida

On Jul 6, 2009, at 2:10 PM, Moody, Pamela wrote:

> Hi Everyone,
> Our core facility has an Aria I, Aria II and LSRII which are all  
> running
> CS&T beads.  We have trained users which are allowed to run these
> instruments and run the CS&T beads.  Each of these users has a  
> different
> lot number of CS&T beads.  I would like to know what other core
> facilities do in this situation.  Can you run different bead lots with
> the same baseline?  Do you ignore the bead lot?  New users need to do
> everything by the book and exactly how they were shown in class.  They
> don't always know what it means if you ask them but they want to do it
> anyway.  I could go on forever about this but I would rather hear your
> stories.
> Thanks
> Pam
> Pamela Moody
> Manager, Flow Cytometry Shared Resource
> Cold Spring Harbor Laboratory
> Hershey Building
> One Bungtown Road
> Cold Spring Harbor, New York 11724
> Phone: (516) 367-8477
> Fax: (516) 367-8461
> Website: http://www.cshl.edu/cancercenter/shared_resources.html
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