[Cytometry] Compensation with liver cells

Mario Roederer roederer at drmr.com
Fri Jul 10 07:29:41 EDT 2009


No... antibody capture beads are not designed with a particular cell  
subset in mind.  Any compensation bead will work for any cell type, as  
long as the positive beads are brighter (or at least as bright) as  
your stained cells.

Adding background to your beads (or using beads with more background)  
will only decrease the precision (although not the accuracy) of your  
compensation.

mr


On Jul 10, 2009, at 5:24 AM, SIMON MONARD wrote:

> You will likely find them unusable as the commercial ones are designed
> for use with lymphocytes. If you are planning on doing much FACS then
> I suggest you make your own capture beads using beads with a
> background fluorescence comparable to your unstained cells



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