[Cytometry] Detecting intracellular human TLRs

María Candela Iglesias Chiesa candela.iglesias at cieni.org.mx
Wed Jul 8 17:50:28 EDT 2009


Dear flowers,

A collegue wants to detect human intracellular TLRs. She bought
fluorochrome-conjugated antibodies from Ebiosciences (TLR3-PE ref. 12-9039
Clone TLR3.7; TLR9-PE, ref. 12-9099, Clone eB72-1665) and is trying to do an
intracellular staining on fresh PBMC, bur results are not great and she
seems to have a very high background fluorescence, even after following
advice from Ebisociences technical support. If somebody has experience with
these antibodies, we welcome any tips.
Thanks!

Candela
Maria Candela Iglesias Chiesa, PhD
Centro de Investigacion en Enfermedades Infecciosas
Instituto Nacional de Enfermedades Respiratorias
Calz. De Tlalpan 4502. Col. Seccion XVI
Deleg. Tlalpan, Mexico DF, Mexico
Tel y fax. +52 55 5666.7985
candela.iglesias at cieni.org.mx
www.cieni.org.mx




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