[Cytometry] adherent cells staining

hanifi ah200259ha at yahoo.com
Wed Jul 1 12:29:05 EDT 2009

Hi everyone,

I have faced problems with staining adherent cells and measuring phospho protein by flow.
we have tried to use the cell lifting buffer(by hyclone) after stimulating cells. we can't see any activation of phospho-protein.
 I was wondering if anyone has done phospho protein staining in adherent cells(like A549/BEAS2B/NIHT3) and seen the activation. if so could you share the protocol you use.

Any suggestion is appreciated.


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