[Cytometry] Drifting on propidium iodide samples

Ray Hicks rhicks at cytekdev.com
Wed Jan 28 17:43:58 EST 2009


Hi Jane,

This kind of behavior can often be put down to residual ethanol remaining in
the sample, which disturbs the hydrodynamic focusing - you could check the
user's protocol and if necessary get them to try washing the cells in
staining medium then re-running them,

Cheers

Ray

Ray Hicks
 
http://www.cytekdev.com

Tel: +44 (0)208 1337 968  Fax: +44(0)208 5889 004  Skype: ray.hicks.cytek 

Cytek Development Europe, Unit 8 The Maltings, Millfield, Cottenham,
Cambridge CB24 8RE. UK



-----Original Message-----
From: cytometry-bounces at lists.purdue.edu
[mailto:cytometry-bounces at lists.purdue.edu] On Behalf Of Miller, Jane S.
Sent: 27 January 2009 14:05
To: cytometry at lists.purdue.edu
Subject: [Cytometry] Drifting on propidium iodide samples

Dear flowers,  Recently in running samples for a client, I noticed that
forward scatter and FL2 fluorescence of PI stained  cells would move to
the right on histograms.  In other words, it appeared smaller cells with
lower PI staining would accumulate first and then larger cells with more
red fluorescence would accumulate.  Has anyone had this happen to them
and what would cause it?  Thanks for any insight.

 

Jane Miller

SRPH 209 Bldg. B

Dept. Micro. Mole. Path.

College of Medicine

Texas A&M Hlth. Sci. Ctr.

College Station, TX  77843

979-862-1376

 

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