[Cytometry] Clinical assistance with an unclassified Non-Hodgkin Lymphoma

Tytherleigh, Lynette Lynette.Tytherleigh at health.wa.gov.au
Wed Feb 25 21:50:08 EST 2009

We have a patient who presented with pancytopenia for investigation. The bone marrow was a dry tap. No fragments were readily visible on the touch preparation of the trephine. However there was a small population of lymphoid cells with clefted nuclei suggestive of a lymphproliferative disorder. 

Immunoperoxidase stains on the trephine showed the lymphoid cells to be positive for CD45RB and CD20. The neoplastic cells were negative for CD5, CD10 and CD23. 50% of the cells were BCL2 positive. Cyclin-D1 was negative. 

Flow Cytometry of bloody aspirate showed 43% of the cells were lymphoid. Of these 33% were T cells and 64% were B cells expressing CD19, CD20 and CD79b with Kappa light chain restriction. 46% of the B cell expressed CD23. The B cells were negative for CD5, CD10 and CD11c.

A sample was sent for Cytogenetic analysis and there was no chromosomal abnormality detected.

Lynette Tytherleigh

Scientist in Charge 
Flow Cytometry Unit
PathWest Laboratory Medicine
Haematology Department, Fremantle Hospital
Alma St, Fremantle 6959
Western Australia
Phone: 08 9431 2459
Fax: 08 9431 2881
Email: Lynette.Tytherleigh at health.wa.gov.au

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