[Cytometry] AlexaFluor 700 on a LSR2?

Tomáš Kalina tomas.kalina at lfmotol.cuni.cz
Mon Feb 23 03:01:35 EST 2009


Hello Michelle,

we use Alexa700 all the time on LSR2, it is excited very well!
It is not so obvious from the excitation curve, but it does work 
perfectly well. You need 685LP and 710/50 filter for collection.
There is considerable overlap from APC into that channel, but Alexa700 
is much more easy to work with than the APC-Cy7, APC-A750 or APC-H7 
tandems. They all require reagent specific compensation and has some 
(sometimes huge) spillover into APC. Moreover APC-Cy7 is quite unstable.

Best regards Tomas

Michelle Tourigny napsal(a):
> Hello all,
> Has anyone used Alexafluor 700 dye on the red laser of the LSR2?  Am I
> correct to assume that it would not be optimally excited?  But you would
> be able to detect any emissions?
> Thanks
> michelle
>
> Michelle R. Tourigny, Ph.D.
> Research Assistant, Instrument Specialist
> Telethon Institute for Child Health Research
> 100 Roberts Road, Subiaco
> Western Australia, 6008 AUSTRALIA
>
> Ph 61 8 9489 7878, Fax 61 8 9489 7700
> email michellet at ichr.uwa.edu.au 
>
>
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-- 
MUDr. Tomáš Kalina, Ph.D.
CLIP - Cytometrie
Klinika dětské hematologie a onkologie
Universita Karlova - 2. lékařská fakulta

Skype: tomaskalina
fax (+420) 22443 6521 (6417)
http://www.lf2.cuni.cz/clip/
tel: (+420) 22443 6477
adresa:       	V Úvalu 84
       		150 06 Praha 5

***********************************************

Tomas Kalina, M.D., Ph.D.
CLIP - Cytometry
Department of Paediatric Hematology/Oncology
2nd Medical School  
Charles University Prague

Skype: tomaskalina
fax (+420) 22443 6521 (6417)
http://www.lf2.cuni.cz/clip/
phone (+420) 22443 6477
postal address:       V Uvalu 84
                      150 06 Praha 5
                      Czech Republic



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