[Cytometry] cytometer - analysis & sorting
Wilhelm.Gerner at vu-wien.ac.at
Sat Feb 21 05:53:35 EST 2009
we are exactly in the situation that you envision: we have an Aria (and
only an Aria) that we use about 80% of its time for analyses and about
20% for sorting. It works well if you have a skilled person around who
knows how to check the machine for proper set-up (needs about 5 minutes
daily). This person should also be around somewhere in the background
when the average user (and especially beginners) analyzes samples
because a sudden clogging might occur and it needs quite a while to
train people how to deal with a clogged nozzle.
So if you can't make sure that such a "supervisor" will be always around
(it works quite well if you split this between two people) than I would
recommend you to purchase an analyzer and do your sorting in a core
facility if available.
Institute of Immunology
University of Veterinary Medicine Vienna
From: cytometry-bounces at lists.purdue.edu
[mailto:cytometry-bounces at lists.purdue.edu] On Behalf Of Gabriele Grunig
Sent: Saturday, February 21, 2009 3:09 AM
To: cytometry at lists.purdue.edu
Subject: [Cytometry] cytometer - analysis & sorting
We are preparing an equipment grant. Ideally, we would need a flow
cytometer that is a good, robust analyzer and that can also be used as a
sorter. I know about the Aria, but am concerned that when this
instrument is used simply as an analyzer it will be too difficult to
operate for people who are occasional users. I am also concerned that
unlike a robust analyzer (e.g. Calibur), a high-speed sorter - even in
the analysis mode - is not going to tolerate cell suspensions that might
have a few clumps.
Is it safe to say that we have to make a choice between an analyzer and
a sorter ? For us the analyzer is more important because it will be
used much more frequently.
Thank you so much for your input !
Gabriele Grunig, DVM, PhD
Department of Environmental Medicine
NYU School of Medicine
57 Old Forge Rd.
Tuxedo NY 10987
email: grunig01 at nyu.edu
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