[Cytometry] Indo-1 on an Aria?
ian.dimmick at newcastle.ac.uk
Fri Feb 20 16:00:34 EST 2009
I have an iCyte UV (355) 60mw laser on my Aria and LSR II , they work well for calcium, however the only problem with the aria is the time it takes for the tube to be ejceted, agonist added, then re introduced to the Aria , this can copromise the calcium assay prportional to the kinetics of the agonist
Flow Cytometry Core Facility Manager
Institute of Human Genetics
International Centre for life
Newcastle Upon Tyne
Ian.Dimmick at ncl.ac.uk
Tel 0044 191 2418831
Fax 0044 191 2418666
(mob) 0044 7970344823
From: cytometry-bounces at lists.purdue.edu [cytometry-bounces at lists.purdue.edu] On Behalf Of Howard Shapiro [hms at shapirolab.com]
Sent: 20 February 2009 20:27
To: Ray Hester
Cc: Cytometry Mailing List
Subject: Re: [Cytometry] Indo-1 on an Aria?
Ray Hester wrote:
> Has anyone demonstrated calcium flux on a FACS Aria or Aria II (_not_ a
> FACSVantage) where at some point after equilibration of Indo1-loaded
> cells, the sample tube was removed from the sample uptake tube, an
> agonist (or some compund) added to the tube, the sample tube replaced
> and the experiment continued?
> If so, or if you know where this type of experiment on an Aria has been
> published, can you point us to references (journal articles) detailing
> the experiment/s?
The major problem with doing calcium flux with indo-1 on most Arias is
that indo-1 won't work - at all! - with either a 405 nm or a 375 nm
laser; you'd have to have a 355 (technically, a 325 nm HeCd would work
but I doubt one has ever been mounted in an Aria).
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