[Cytometry] Indo-1 on an Aria?

Alice L. Givan Alice.L.Givan at Dartmouth.EDU
Fri Feb 20 16:04:59 EST 2009


Nick Goulding did flow calcium ion analysis in our lab a long time ago.  He developed a method for hanging a drop of the agonist on the side of the sample tube -- in such a way that it would only be released into the cell suspension when the tube was vortexed.

So -- put your cell suspension into a tube,
  hang the agonist solution on the side of the tube, 
 load the tube into the cytometer, 
start taking a minute or so of base line flow data,  
and then vortex the tube in place to start the calcium flux reaction.

Alice

Alice L. Givan, Director
Englert Cell Analysis Laboratory
of the Norris Cotton Cancer Center
Dartmouth Medical School
Lebanon, NH 03756 USA
tel 603-650-7661
fax 603-650-6130
givan at dartmouth.edu
www.dartmouth.edu/~celllab


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