[Cytometry] Indo-1 on an Aria?

Ray Hester rhester at jaguar1.usouthal.edu
Thu Feb 19 10:58:33 EST 2009


Hi,

Has anyone demonstrated calcium flux on a FACS Aria or Aria II (_not_ a 
FACSVantage) where at some point after equilibration of Indo1-loaded 
cells, the sample tube was removed from the sample uptake tube, an 
agonist (or some compund) added to the tube, the sample tube replaced 
and the experiment continued?

If so, or if you know where this type of experiment on an Aria has been 
published, can you point us to references (journal articles) detailing 
the experiment/s?

Thanks.

Ray Hester
Research Cytometry Laboratory
College of Medicine
The University of South Alabama
Mobile, AL 36688


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