[Cytometry] cloning smoothly?

Michelle Tourigny michellet at ichr.uwa.edu.au
Tue Feb 17 21:15:43 EST 2009


When you are doing cloning work into 96 well plates and human samples is
it possible to use the AMO?  Does that affect the positioning of the
cells?
Thanks
Michelle

Michelle R. Tourigny, Ph.D.
Research Assistant, Instrument Specialist
Telethon Institute for Child Health Research
100 Roberts Road, Subiaco
Western Australia, 6008 AUSTRALIA

Ph 61 8 9489 7878, Fax 61 8 9489 7700
email michellet at ichr.uwa.edu.au 

-----Original Message-----
From: cytometry-bounces at lists.purdue.edu
[mailto:cytometry-bounces at lists.purdue.edu] On Behalf Of Perry, Greg
Sent: Tuesday, 17 February 2009 12:15 AM
To: Ray Hester; Cytometry Mailing List
Subject: Re: [Cytometry] cloning smoothly?

Ray,

We clone frequently with our Aria.  Works fine.  Certainly the jerky
ride isn't enough to spill any of the cells out of even full wells in a
96-well plate.  In addition, we've put cells into the Advalytix wells
and had no problems with cells or media "spilling over" out onto the
rest of the slide.

Is the MoFlo smoother?  I don't know.  Does it matter?  Not in my
experience.

- Greg
 
Greg A. Perry, Ph.D.
Technical Director
Flow Cytometry Core Facility
Creighton University
Omaha, NE  68178
(402) 280-1841
www2.creighton.edu/medschool/medicine/cytometry



-----Original Message-----
From: cytometry-bounces at lists.purdue.edu
[mailto:cytometry-bounces at lists.purdue.edu] On Behalf Of Ray Hester
Sent: Friday, February 13, 2009 10:01 AM
To: Cytometry Mailing List
Subject: [Cytometry] cloning smoothly?

Hi,

We were wondering if anyone has experience with cloning using both an
AriaII and a MoFlo XDP?  Specifically, is the MoFlo a smoother 'ride' 
from well to well?  Watching a video from Advalytix (no financial
interest) of single-cell deposition onto an AmpliGrid slide using the
Aria, at:

http://www.advalytix.com/single_cell_amplification_1333.htm

it seems the motion from well to well is not excessively jerky as we've
been told it is - I mean, it has to stop and start at each well, right? 
In comparison, is cloning on the MoFlo a much smoother operation than
what's seen in the video at the above link?

Thanks for any thoughts on this.

Ray Hester
College of Medicine
Univ. of South Alabama




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