[Cytometry] Violet laser for LSR2 - are there better options?

Telford, William (NIH/NCI) [E] telfordw at mail.nih.gov
Tue Feb 17 00:57:08 EST 2009

Hi Tomas...

I think most of the instrument manufacturers are using at least a 50 mW violet laser now,  and 100 mW versions are available.  If your supplier offering you the 25 mW version, ask for a more powerful one - most of the major violet laser suppliers offer their units in standardized housings, so it it not a big deal to add a more powerful unit, and the cost increase should not be much.

In our hands, more power is definitely better for violet-excited probes, especially photon-demanding ones like CFP FRET.  So get the most powerful module you can.  Also, if you are ordering a cytometer with fiber-coupled lasers (Aria II, Canto II, etc.), a lot of power is lost in the fiber - again, start with the most powerful laser you can.



-----Original Message-----
From: Tomás Kalina [mailto:tomas.kalina at lfmotol.cuni.cz]
Sent: Mon 2/16/2009 3:42 AM
To: Cytometry at lists.purdue.edu
Subject: [Cytometry] Violet laser for LSR2 - are there better options?
Dear flow colleagues,

can you recommend a better choice of the Violet laser for excitation of 
Pacific Blue, Pacific Orange and Qdot605 then the standard 405nm 25mW 

What I see is a lower intensity and resolution of the Pacific Blue as 
compared to the same sample measured on the Cyan ADP with 100mw Coherent 
compass 405nm.

Thank you for any suggestions,

MUDr. Tomás Kalina, Ph.D.
CLIP - Cytometrie
Klinika detské hematologie a onkologie
Universita Karlova - 2. lékarská fakulta

Skype: tomaskalina
fax (+420) 22443 6521 (6417)
tel: (+420) 22443 6477
adresa:       	V Úvalu 84
       		150 06 Praha 5


Tomas Kalina, M.D., Ph.D.
CLIP - Cytometry
Department of Paediatric Hematology/Oncology
2nd Medical School  
Charles University Prague

Skype: tomaskalina
fax (+420) 22443 6521 (6417)
phone (+420) 22443 6477
postal address:       V Uvalu 84
                      150 06 Praha 5
                      Czech Republic

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