[Cytometry] Human vs Mouse markers (xenografted cells)

Rachel Gerstein rachel.gerstein at umassmed.edu
Fri Feb 13 14:10:22 EST 2009


They should check whether the tumor cells still express MHC Class I.  There
are human-specific and mouse-specific antibodies for Class I.


On 2/11/09 5:46 PM, "Christiane Hassel" <chassel at indiana.edu> wrote:

> Hi there,
> 
>   I have had an inquiry from one of the researchers who utilize the flow
> facility.  We haven't run any samples from xenograft studies (that I can
> recall), therefore I was wondering if someone could help me answer their
> question.  Basically, how to ID and separate human vs mouse cells in the
> case of xenograft.
> 
> Their inquiry:
> 
> "I have sets of cells that are derived from breast cancer tumors grown
> in a mouse, and would like to verify that my culture is ONLY the human
> injected cells from the tumor and that I do not have any mouse stromal
> cells that have survived my antibiotic and drug selection methods."
> 
> Any help is greatly appreciated!
> 
> Christiane
> 
> Christiane Hassel, B.Sc.
> FACS Operator/Manager
> IU Bloomington
> Flow Cytometry Core Facility
> Jordan Hall 029
> http://facs.bio.indiana.edu/
> 812-855-7101
> chassel at indiana.edu
> 
> 
> 
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