[Cytometry] Conjugation of antibodies with tandem dyes

Guy Hermans Guy.Hermans at ablynx.com
Tue Feb 10 10:58:38 EST 2009


Hi J.,

These have been mentioned a few times here already, always favorably.
That includes my own experiences ;-)

I do have to mention I never played with their tandem conjugation kits,
just the single fluors. I'd be happy to hear about other users'
experience on that level as well.

Cheers,

Guy


-----Original Message-----
From: cytometry-bounces at lists.purdue.edu
[mailto:cytometry-bounces at lists.purdue.edu] On Behalf Of Parmentier,
Julie
Sent: Tuesday, February 10, 2009 12:34 AM
To: cytometry at lists.purdue.edu
Subject: [Cytometry] Conjugation of antibodies with tandem dyes

Hello everyone!

I'm looking to conjugate some antibodies to tandems (Cy5-PE, Cy7-PE, and
Cy7-APC), and was wondering if anyone has any experience with Innova
Biosciences "Lightning-Link" conjugation kits.  I've visited Dr. Mario's
site for his advice, and found a great protocol that would absolutely be
my first choice, except who can resist "The World's Easiest Conjugation
Kits".  I do love easy, and more than that, I love a kit.  But I am
worried that "easiest" will not translate into "best", or even
"good-enough".  So before I pin my hopes on this, has anyone tried it
out, and if you have, how did it work?? Any tips/advice would be greatly
appreciated..If no one has tried it out I'd be willing to take a shot at
it, see how it goes,and let you know how it turns out.....Thanks
everyone!


Julie Parmentier
Scientist
Cancer Biologics
AstraZeneca
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