[Cytometry] Whole Blood Samples VS Cyan ADP
t.adejumo at ucl.ac.uk
Mon Feb 9 11:30:18 EST 2009
We currently use a Cyan ADP from Beckman Coulter and one of my
colleagues is analysing blood samples and prepares the cells using a
lysing kit and protocol, e.g.
60ul Formic acid.
10% Formaldehyde (from paraformaldehye solid) in Reagent 'B'
Use all reagents at 0'C
i) Add 5ul ab/50ul whole blood
ii) Incubate on ice 20mins
iii) Add 300ul 'A' -Vortex/10 seconds.
iv) Add 132.5ul 'B' -Vortex/10 seconds.
v) Add 50ul 'C' -Vortex/10 seconds.
> Keep samples in the 'fridge' or on ice -should be stable for around a
> Run on the flow.
However, on running the samples, some of them appear to block the SIP of
the Cyan. We have checked for pressure problems etc but there are none.
And when running some of her samples, she doesn't experience this. The
user doesn't wish to filter the samples but does use EDTA and heparin.
When running the same sample on the FACS Calibur, she doesn't notice
Can you suggest why that is?
Tomas Adejumo MSc
Applications Specialist - Flow Cytometry and Confocal Microscopy
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