[Cytometry] Help with macrophage detachment

McClellan, Steve smcclell at UFL.EDU
Fri Feb 6 11:40:47 EST 2009

You are right Mario, the ACAS dates back to the late 80s/early 90s  from Meridian.  It did have in situ ablation capabilities, but you had to manually target the cells, like a video game. It was a very cool back in the day.  This new system, LEAP from Cyntellect is light years ahead in terms of being fully automated.  We have one here at UF and are exploring many novel uses, both for adherent cell sorting and the laser mediated optoinjection modes.  We are very impressed with it.

Steve McClellan
Sr. Biological Scientist
Interdiciplinary Center for Biotechnology Research(ICBR)-
Flow Cytometry Core Laboratory
University of Florida
352-273-8185 (office)
352-273-8070 (fax)
smcclell at biotech.ufl.edu


-----Original Message-----
From: cytometry-bounces at lists.purdue.edu [mailto:cytometry-bounces at lists.purdue.edu] On Behalf Of Mario Roederer
Sent: Friday, February 06, 2009 11:13 AM
To: Baggi Somasundaram
Cc: cytometry at lists.purdue.edu
Subject: Re: [Cytometry] Help with macrophage detachment


Wasn't there a whole company built around this principle?  The ACAS  
470?  Some 10-15 years ago.  This is so old I can't even remember the  
details any more...


On Feb 5, 2009, at 1:37 PM, Baggi Somasundaram wrote:

> A novel technology exists for purification of adherent cells which  
> allows cells to be analyzed and purified in situ rapidly without  
> having to remove the cells from their substrates (multiwall plates).  
> This In Situ Cell Purification process technology enables rapid  
> generation of pure live cell populations from very small cell  
> samples ranging from 2 to 1000 's of cells based on functional or  
> cell marker based characteristics.
> The original work was published in Cytometry 61A:153-161 (2004) High- 
> Throughput Laser -Mediated In situ Cell purification with high  
> purity yield. Manfred R. Koller et al.
> Adherent cells can be purified without forcing them into suspension,  
> thus preserving both their physiological and structural integrity  
> and cell health. It is accomplished by using ultra fast (1000 cells/ 
> sec) laser manipulation to selectively and efficiently eliminate  
> undesired cells.  This non invasive In Situ process allows sparse  
> cell populations including primary cells and stem cells to be  
> purified in their closed sterile incubation environment with very  
> high yield in less than half the time compared to other conventional  
> techniques.
> This purification process compliments FACS systems and is suited for  
> all cell types, including adherent cells, non-adherent cells,  
> primary cells, Embryonic & iP stem cells, cancer stem cells, rare  
> cells and fragile cells.
> Baggi Somasundaram
> bsomasundaram at cyntellect.com
> _______________________________________________
> Cytometry mailing list
> Cytometry at lists.purdue.edu
> https://lists.purdue.edu/mailman/listinfo/cytometry

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