[Cytometry] Help with macrophage detachment
roederer at drmr.com
Fri Feb 6 11:13:17 EST 2009
Wasn't there a whole company built around this principle? The ACAS
470? Some 10-15 years ago. This is so old I can't even remember the
details any more...
On Feb 5, 2009, at 1:37 PM, Baggi Somasundaram wrote:
> A novel technology exists for purification of adherent cells which
> allows cells to be analyzed and purified in situ rapidly without
> having to remove the cells from their substrates (multiwall plates).
> This In Situ Cell Purification process technology enables rapid
> generation of pure live cell populations from very small cell
> samples ranging from 2 to 1000 's of cells based on functional or
> cell marker based characteristics.
> The original work was published in Cytometry 61A:153-161 (2004) High-
> Throughput Laser -Mediated In situ Cell purification with high
> purity yield. Manfred R. Koller et al.
> Adherent cells can be purified without forcing them into suspension,
> thus preserving both their physiological and structural integrity
> and cell health. It is accomplished by using ultra fast (1000 cells/
> sec) laser manipulation to selectively and efficiently eliminate
> undesired cells. This non invasive In Situ process allows sparse
> cell populations including primary cells and stem cells to be
> purified in their closed sterile incubation environment with very
> high yield in less than half the time compared to other conventional
> This purification process compliments FACS systems and is suited for
> all cell types, including adherent cells, non-adherent cells,
> primary cells, Embryonic & iP stem cells, cancer stem cells, rare
> cells and fragile cells.
> Baggi Somasundaram
> bsomasundaram at cyntellect.com
> Cytometry mailing list
> Cytometry at lists.purdue.edu
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