[Cytometry] Help with macrophage detachment

Mario Roederer roederer at drmr.com
Fri Feb 6 11:13:17 EST 2009


Wasn't there a whole company built around this principle?  The ACAS  
470?  Some 10-15 years ago.  This is so old I can't even remember the  
details any more...


On Feb 5, 2009, at 1:37 PM, Baggi Somasundaram wrote:

> A novel technology exists for purification of adherent cells which  
> allows cells to be analyzed and purified in situ rapidly without  
> having to remove the cells from their substrates (multiwall plates).  
> This In Situ Cell Purification process technology enables rapid  
> generation of pure live cell populations from very small cell  
> samples ranging from 2 to 1000 's of cells based on functional or  
> cell marker based characteristics.
> The original work was published in Cytometry 61A:153-161 (2004) High- 
> Throughput Laser -Mediated In situ Cell purification with high  
> purity yield. Manfred R. Koller et al.
> Adherent cells can be purified without forcing them into suspension,  
> thus preserving both their physiological and structural integrity  
> and cell health. It is accomplished by using ultra fast (1000 cells/ 
> sec) laser manipulation to selectively and efficiently eliminate  
> undesired cells.  This non invasive In Situ process allows sparse  
> cell populations including primary cells and stem cells to be  
> purified in their closed sterile incubation environment with very  
> high yield in less than half the time compared to other conventional  
> techniques.
> This purification process compliments FACS systems and is suited for  
> all cell types, including adherent cells, non-adherent cells,  
> primary cells, Embryonic & iP stem cells, cancer stem cells, rare  
> cells and fragile cells.
> Baggi Somasundaram
> bsomasundaram at cyntellect.com
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