[Cytometry] Help with macrophage detachment

Dr Neil Farrow n.farrow at med.keele.ac.uk
Thu Feb 5 09:38:44 EST 2009

Gentle scraping was the way I was taught in a lab with much experience
with hmdm's. I'm sure if another method for detachment was available they
would have used it.

Dr Neil Farrow
nanoTherics Ltd

Company No: 6332067 registered in England and Wales.

Guy Hilton Research Centre
School of Medicine
Keele University
Thornburrow Drive
United Kingdom
Tel 01782 554 047
Fax 01782 747 319
email neil at nanoTherics.com

> Have you tried culturing in petri dishes (you know the common round (or
> square) ones used for microbiology culture)? They are sterile, the MACs
> attach but then easily come off easily with a gentle tap. It was a long
> time ago but you may need bring to room temp prior, too.
> I believe the other tissue culture plates are coated to aid attachment.
> Good luck
> Natalie
> Natalie Parlane
> Research associate
> Infectious diseases
> Hopkirk Research Institute
> AgResearch
> New Zealand
> T +64 6 351 8692
> F +64 6 353 7853
> E natalie.parlane at agresearch.co.nz
> -----Original Message-----
> From: cytometry-bounces at lists.purdue.edu
> [mailto:cytometry-bounces at lists.purdue.edu] On Behalf Of Wiener, Doris
> Sent: Saturday, 31 January 2009 4:15 a.m.
> To: cytometry at lists.purdue.edu
> Subject: [Cytometry] Help with macrophage detachment
> Thank you in advance for your help with this issue.
> I am trying to detach human monocyte derived macrophages from tissue
> culture dishes. I have tried Sigma's Cell Detachment Media and Genlantis'
> Detachin and have considerable cell death and up to 20% of the cells still
> remain on the plate. I am hesitant to use trypsin/EDTA as I need to do
> surface staining of the cells for flow cytometry. I have also tried
> mechanical removal (gentle scraping) but that also caused a high
> percentage of dead cells. Thanks
> Doris Wiener
> University of South Florida
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