[Cytometry] PMA/ionomycin and PI

Pierre, Melissa MPIERRE19 at PARTNERS.ORG
Tue Feb 3 08:50:23 EST 2009

 Our lab is selling our Beckman Coulter FC500 Flow Cytometer.  Is anyone

Melissa Pierre, BA 
Clinical Research Coordinator 
Massachusetts General Hospital 
Immunology Lab - Diabetes Research
CNY, 13th Street, Building 149, Room 3603 
Charlestown, MA 02129 
Phone: 617-726-4084 

-----Original Message-----
From: cytometry-bounces at lists.purdue.edu
[mailto:cytometry-bounces at lists.purdue.edu] On Behalf Of Monica Spadafora
Sent: Monday, February 02, 2009 11:48 AM
To: cytometry at lists.purdue.edu
Subject: [Cytometry] PMA/ionomycin and PI

Dear all,

I would like to know if there is any interference of PMA and ionomycin on
measuring cell 
viability with propidium iodide.
I observed that after 24h incubation of mouse esplenocytes with PMA and
ionomycin all 
cells became PI positive in contrast to unstimulated cells.

Any help will be appreciated

Monica Spadafora Ferreira
Butantan Institute
Cytometry mailing list
Cytometry at lists.purdue.edu

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