[Cytometry] PMA/ionomycin and PI

Monica Spadafora Ferreira mospadafora at butantan.gov.br
Mon Feb 2 11:47:50 EST 2009

Dear all,

I would like to know if there is any interference of PMA and ionomycin on measuring cell 
viability with propidium iodide.
I observed that after 24h incubation of mouse esplenocytes with PMA and ionomycin all 
cells became PI positive in contrast to unstimulated cells.

Any help will be appreciated

Monica Spadafora Ferreira
Butantan Institute

More information about the Cytometry mailing list