[Cytometry] PMA/ionomycin and PI

Monica Spadafora Ferreira mospadafora at butantan.gov.br
Mon Feb 2 11:47:50 EST 2009


Dear all,

I would like to know if there is any interference of PMA and ionomycin on measuring cell 
viability with propidium iodide.
I observed that after 24h incubation of mouse esplenocytes with PMA and ionomycin all 
cells became PI positive in contrast to unstimulated cells.

Any help will be appreciated
Regards

--
Monica Spadafora Ferreira
Butantan Institute
Brasil


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