[Cytometry] 5 color compensation issue

Zhugong Liu zliu at immunetolerance.org
Mon Feb 2 22:29:26 EST 2009


Dear all,

We are running 5-color flow on FC500 and have some compensation issues.
The 5-color cocktail includes antibodies with the following
fluorechromes: FITC, PE, PE-Cy5.5, PE-Cy7 and APC. Do you think there
might be some reagent interactions in this panel combo, especially
PE-Cy5.5 and other dyes? Our single controls were well compensated using
the software-generated matrices, however the fully stained samples were
not. 

Thanks for any comment/suggestion.

Best,
Jason

-------------------------------------------------------
Jason Liu, PhD
Asst. Director, Flow Cytometry
Tolerance Assays and Data Analysis
UCSF/Immune Tolerance Network
3 Bethesda Metro Center, Suite 400
Bethesda, MD 20814
Phone: 240-235-6177
Fax: 240-235-6198
Email: zliu at immunetolerance.org



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