[Cytometry] 532 vs 568 laser

Christopher Norbury ccn1 at psu.edu
Sun Feb 1 10:09:33 EST 2009


We are hoping to begin purchase of a new 4 laser instrument in the near
future.  We've settled on 3 lasers to include, a 405nm near-UV, the standard
488nm and a 633nm.  We are now deciding between a 532nm and 568nm laser for
the mid-ranges.  Could anyone give any input on the applicability of these
lasers for various dyes (PE, PE tandem conjugates, fruity dyes (mCherry,
tomato etc), RFP etc?  Also, is there any major difference in the
reliability of these two respective lasers?  Finally, any information on the
compensation issues of the 532 with the 488 and the 568 with the 633 would
be much appreciated.



Chris Norbury, Ph.D
Associate Professor of Microbiology and Immunology
Scientific Director, Flow Cytometry Core Facility.
Penn State Milton S. Hershey College of Medicine
Room C6804A, Dept. of Microbiology and Immunology, H107
500 University Drive, Hershey, PA 17033-2360
Lab      717 531 0624
Office   717 531 7204
Fax      717 531 6522
Email    ccn1 at psu.edu
Web      http://fred.psu.edu/ds/retrieve/fred/investigator/ccn1

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