[Cytometry] 32nd Annual Course in Flow Cytometry - second announcement

Claire Sanders csanders at lanl.gov
Wed Apr 15 18:41:05 EDT 2009


32nd ANNUAL RESEARCH COURSE IN FLOW CYTOMETRY


Albuquerque, New Mexico, May 30-June 5, 2009



Sponsored by:  The National Flow Cytometry Resource (NFCR) and Verity
Software House 

Hosted by:  The Center for Biomedical Engineering at the University of New
Mexico

Course Description:  A methods course in flow cytometry will be offered with
special emphasis on applications in cell biology, immunology and screening.
The faculty consists of distinguished members of the flow cytometry
community.  This course will be aimed toward individuals with several years
of experience in flow cytometry; very little basic material will be
presented.  Each participant will receive extensive lecture notes and
laboratory hand-out materials.


 

Title

Lecturer


1

Basics of Flow Cytometry

John Martin


2

Fluorescent Probes and Fluorescence Detection

Alan Waggoner


3

Data Analysis

Jim Jett


4

DNA Analysis

Alice Given


5

Analysis and Display of High Dimensional Data 

Bruce Bagwell


6

Sorting

Joe Trotter


7

Fluorescent Proteins in Flow and Imaging

David Galbraith


8

Apoptosis

Bill Telford


9

Strategies in Multicolor Flow Cytometry

Carlton Stewart


10

Analysis of Intracellular Epitopes

Vince Shankey


11

Molecular Oncology

David Hedley


12

Flow Cytometry Instrument Calibration

James Wood


13

Sensitivity in Flow Cytometry

Bob Hoffman


14

Time, Biochemistry and Cell States

James Jacobberger


15

Environmental Metagenomics

Cheryl Kuske


16

Engineering Fluorescent Proteins as Ligands

Andrew Bradbury


17

Raman Spectral Flow Cytometry

John Nolan


18

The Future of Flow Cytometry Instrumentation

Steve Graves


Evening session 1: Instrument Operation in the Real World

Kevin Holmes: Biosafety in Flow Cytometry

Joe Trotter & Bill Telford: Issues with Running a Flow Cytometry Core
Facility

Evening session 2: Highlighting Instrument Development at the NFCR

Jessica Houston:  Fluorescence Lifetime Measurement

Greg Goddard:  High Resolution Spectral Flow Cytometry

Robert Applegate:  High-Throughput Large Particle Analysis


Special Invited Lecture:  

Dr. Garry Nolan - Baxter Laboratory in Genetic Pharmacology Stanford
University

Multiparameter Phosphoprotein Analysis in Single Cells by Flow Cytometry

Laboratory sessions will address sample preparation, instrument
construction, data collection and analysis, common and more cutting edge
flow assays, and typical problems encountered in flow cytometry.  Lab
sessions will be hands-on, with plenty of time and opportunity for
interaction with the instructors.  Lab sessions will utilize a variety of
commercial and developmental instruments, and representatives from several
of the commercial vendors will be available to demonstrate and discuss their
most recent instruments and techniques.  Each participant will be given the
choice of attending six laboratory sessions.


 

Lab

Instructors


1

Build It

John Martin, Mark Wilder, Mark Naivar, Jim Jett


2

Displaying and analyzing high dimensional data using probability state
modeling

C. Bruce Bagwell


3

DNA analysis

Alice Givan


4

Immunophenotyping

Carlton Stewart, Sigie Stewart


5

Intracellular antigens

Vince Shankey, David Hedley, Sue Chow


6

Apoptosis

Bill Telford


7

Sorting

Joe Trotter


8

High throughput

Bruce Edwards, Larry Sklar


9

Fluorescent protein analysis and sorting

David Galbraith, Teresa Hawley, Georgina Lambert


10

Chromosome analysis

Claire Sanders


11

Advanced cell cycle analysis

Jake Jacobberger, Mike Sramkoski


12

Glow little glow-germ, glow

Kevin Holmes, Steven Perfetto


13

Standardization & calibration

James Wood, Bob Hoffman


14

Bead-based assays

Tione Buranda

The course fee of $1600 includes all lectures and laboratory sessions, a
welcoming reception on Saturday evening, breaks and lunches during the week,
and a banquet on Thursday evening.  Optionally, for a total fee of $1850 you
will also have on-campus housing in apartment style dorms, including buffet
style breakfast in the cafeteria. 

Complete information is available on the NFCR home page at:
http://nfcr.lanl.gov/ <http://nfcr.lanl.gov> .  For more details, questions,
or a registration form please contact Ruby Archuleta (505-667-3912,
rubyra at lanl.gov).

 

Hope to see you there!

 

 

 

Claire K. Sanders

National Flow Cytometry Resource Technician

 

Advanced Measurement Science (B-9)

Bioscience Division

Los Alamos National Laboratory

PO Box 1663, MS M888

Los Alamos, NM 87545

 

Phone: (505) 667-2788

Fax: (505) 665-3024

 



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