Make 30 uM mesh filters
saeed.ghani at gmx.net
Wed Oct 8 17:00:32 EDT 2008
dear brian, dear mara,
an alternative is to reuse the bd filters. after use store them
submersed in water, wash them with soap and a small brush and air dry
the filters. for sterile use they can be autoclaved. this works with
all mesh sizes multiple times.
of note: the cell suspension does not run through the washed filter
immediately: therefore put a drop of pbs/ water on the filter from the
opposite site first.
happy single cell preparation.
Am 08.10.2008 um 08:46 schrieb Mara Rocchi:
> Dear Brian,
> I use two overlapped pieces of microscope lens filter paper folded in
> a cone shape -similar to the old cones made with filter paper to
> remove debris from dye solution. Put the cone in the tube and filter
> your cells. It will take some time but it does work. This is very
> inexpensive and effective in removing large-to medium clumps, even if
> some two-three cell clumps can remain.
> You can even prepare the cones in advance and get them sterilized.
> Best regards
> Mara Rocchi BVM&S, PhD
> Flow Cytometry Manager
> Moredun Research Institute
> Pentlands Science Park
> Bush Loan, Penicuik
> Scotland, UK
> Think before you print. Save paper and help the environment
> From: Brian McFarlin [mailto:bmcfarlin at mail.coe.uh.edu]
> Sent: 07 October 2008 14:04
> To: Cytometry Mailing List
> Subject: Make 30 uM mesh filters
> Hello All,
> I am working on an experiment where I have been running through a
> large number of 30 uM nylon mesh filters to remove clumps and debris
> from my cell suspensions. I know that I have seen before that some
> individuals have made their own debris filters. I was wondering if
> someone could let me in on how you would do this. I currently am
> filtering into a 15 mL centrifuge tube if that makes any difference.
> Thanks in advance,
> Brian K. McFarlin, PhD
> Assistant Professor Exercise Physiology / Nutrition
> University of Houston
> Department of Health and Human Performance
> 3855 Holman St.
> Houston, Texas 77204-6015
> (713) 743-9929 phone
> (713) 743-9860 fax
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# # #
saeed.ghani at gmx.net
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