Accudrop beads

Vinko Tosevski vinko.tosevski at gmail.com
Sun Nov 16 08:13:58 EST 2008


Hi Betty-Anne,

We used to have such a problem. Actually, some time ago I posted the
same question on the Purdue list
(http://www.cyto.purdue.edu/hmarchiv/2008/thread.htm#546). In our
case, focusing the camera behind the sort chamber (we have Aria I)
really helped. It is explained here
(http://www.cyto.purdue.edu/hmarchiv/2008/0565.htm).

Vinko


2008/11/13 Betty-Anne McBey <bmcbey at ovc.uoguelph.ca>:
> Has anyone every experienced a sum of less than 100% in the two windows when
calibrating
> with Accudrop beads?	Eg 40% in the left sort window and 20% in the stream?  What
should
> I be correcting to get it to add up to 100%?	This happens occasionally with my sorter.
>
> Betty-Anne McBey, AHT, BSc, MSc, CD, Pmsc
> Department of Pathobiology
> Ontario Veterinary College
> University of Guelph
> Guelph, ON
> N1G 2W1
> Telephone 519-824-4120 ext 54758 or 54664
>
>



-- 
Vinko Tosevski, MMolBiol
Research and Teaching Assistant
Department of Physiology and Immunology
Rijeka Medical School

B.Branchetta 20
HR-51000 Rijeka
Croatia

tel: +385 51 651192
fax: +385 51 675699



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