LSRII filters on a CSU-X1 confocal head

Benoit Labarthe benoitlabarthe at
Wed Mar 12 22:51:41 EDT 2008

Hello, flowers and microscopists!

Here is a chimera-type question!

We have emission filters from an BD LSRII flow cytometer (disks of 25mm of
diameter) and would like to use them in a CSU-X1 confocal head. Basically,
the filters are fitting in the emission wheel of the CSU and we see
fluorescence. My question is: do emission filters for flow cytometry and for
confocal microscopy have the same properties?
I may be wrong, but both are the final emission filters in front of either
the PMT or the camera and thus have a similar role of filtering the
emissions. In my mind, both filters should be comparable. Do I omit any
critical detail?

Thank you!


Benoît Labarthe, PharmD
Institut de Cardiologie de Montréal
Montreal Heart Institute
Centre de Recherche, Laboratoire du Dr.Théroux
5000 Bélanger Est
Montréal Québec Canada H1T 1C8
Tel (514)-376 3330 ext 3017
Fax (514)- 376 1076
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