Validation report for multiple staining

Cythéris - Stéphanie BEQ sbeq at
Tue Jun 24 12:35:47 EDT 2008

Dear flowers,


I have just join a private company (I was previously working in the
academic) and one of my topics is to install flow cytometry (not a problem
for research use) by Quality Assurance (that is the problem) and I’m not
familiar with QA. I have already found the Canadian Guideline for flow
cytometry (2001) and the H42-A2 from NCCLS on the web and the daily/monthly
QC of the instrument itself should not represent a big difficulty, but the
validation of staining, antibodies panel, compensation, multicolor analysis,
gating, reproducibility, what kind of calibrator should I choose
unclear for me.

Is anybody having a two (or more of course!) colors staining validation
report (or validation plan)? It could be really helpful for me to adapt it
and develop it for an 8 colors panel.

Does more recent guidelines exist (FDA or CDC or else?) ?

Thank you for your help.


Best regards,


Stéphanie Beq





BEQ Stephanie

Immunological Studies Manager


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 <mailto:bassouline at> sbeq at

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fax 33(0)1 4644 4446




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