Manfra, Denise denise.manfra at SPCORP.COM
Mon Jun 9 16:08:53 EDT 2008

We are trying to access neutrophils and Eosinophils in rat BAL in an OVA
model at 24 and 72 hrs post challenge.

We have identified at least 4 populations

A) Macrophages: CD45Hi, DAPI+/- (pending activation state), SSCInt/Hi,
FSChi, Ox41Hi, Anti-Gran dim/neg

B) Could these be neutrophils?	 CD45Lo/+, SSCHi, Ox41Int, anti-gran
dim, FSC lo,  take up a lot of DAPI: 

C) Identity unknown?  CD45dim/+, SSCverylo, Ox41neg, anti-gran dim/+,
FSC:very small: 

D) Could these be eosinophils?	    CD45+, DAPI neg, SSCHi, FSCInt,
Ox41Int, Anti-Gran dim

E) Could these be DCs?	   CD45Hi, DAPI+/neg, SSCInt, Ox41Int/Hi,

Alum controls: primarily A and C, very few B at 24 hrs or 72 hrs

OVA 24 hrs: Primarily A and B OR D and E, Fewer A, and a few D

OVA 72 hrs: Primarily D, Fewer A and B

This message and any attachments are solely for the
intended recipient. If you are not the intended recipient,
disclosure, copying, use or distribution of the information 
included in this message is prohibited -- Please 
immediately and permanently delete.
-------------- next part --------------
HTML attachment scrubbed and removed

More information about the Cytometry mailing list