How to deal with ARIA contamination?

Alice L. Givan Alice.L.Givan at dartmouth.edu
Wed Jan 30 14:53:57 EST 2008


  Our Aria does not have contamination problems.  This could be just luck.  But our
routine is as follows:

We NEVER run anything through the sheath lines except autoclaved PBS.

We have purchased extra sheath tanks.  We fill them with sheath fluid and autoclave them.
 We switch tanks as neatly as possible when a tank runs out. 

And that's it.

My theory is that there should not be any contamination in the sheath lines if the only
thing that has passed through those lines is sterile PBS.


Alice

Alice L. Givan
Englert Cell Analysis Laboratory
of the Norris Cotton Cancer Center
Dartmouth Medical School
Lebanon, NH 03756 USA
tel 603-650-7661
fax 603-650-6130
givan at dartmouth.edu
www.dartmouth.edu/~celllab




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