Apoptosis staining in whole blood
kfurr at bidmc.harvard.edu
Fri Jan 25 14:25:59 EST 2008
Hi, Our group is trying to develop an assay that will allow us to determine apoptotic and necrotic lymphocytes (primarily apoptotic cells) in rhesus macaque whole blood samples. In the future, we would like to avoid enrichment of lymphocytes by density gradient separation since these procedures can significantly change lymphocyte subset distributions. We are currently using Annexin V and would like to continue to use Annexin V. However, the problem we are running into is how to eliminate RBCs without increasing the affinity for or losing Annexin V binding. Any suggestions as to a procedure that would allow us to reduce RBCs without affecting the integrity of the Annexin V binding or possibly an alternative method to Annexin V staining that will not be affected by the RBC lysis step would be greatly appreciated.
Many thanks in advance,
Division of Viral Pathogenesis
Beth Israel Deaconess Medical Center
41 Avenue Louis Pasteur
Boston, MA 02115
Phone : 617-667-4707
Fax : 617-667-8210
This document may contain information that is privileged, CONFIDENTIAL and exempt from disclosure under applicable law. If you are not the intended recipient, please notify me immediately, as the use of this information is strictly prohibited.
-------------- next part --------------
HTML attachment scrubbed and removed
More information about the Cytometry